Essential oils from needles, fruits and bark were extracted from Pinus pinea L. (stone pine) grown wildly in Jordan. The chemical composition, antibacterial activity, antioxidant activity of essential oils were evaluated. The chemical compositions were identified using Gas-Chromatography-Mass spectrometry (GC_x0002_MS) and retention indices (Van den Dool & Kratz). The results show that the essential oil obtained from nee_x0002_dles composed mainly of guaiol (12.7%), limonene (11.42%), and β-caryophyllene (7.61%), while fruit con_x0002_tains limonene (32.56%), and α-pinene (6.78%). The essential oils from barks were rich in β-caryophyllene (16.51%), limonene (14.83%), caryophyllene oxide (11.83%), and longifolene (7.51%). In vitro, the antibac_x0002_terial activity of the essential oils was evaluated using the agar-well diffusion method against three different strains of bacteria (Gram-positive bacteria: Staphylococcus aureus and Gram-negative bacteria: Klebsiella pneumoniae and Escherichia coli). The results indicated that essential oil exhibited appreciable antibacterial activity against S. aureus. The essential oil from fruit exhibited weak antibacterial activity against E. coli and K. pneumoniae. Essential oils of P. pinea showed appreciable antioxidant activity in-vitro.
